the art and design of genetic screens: caenorhabditis elegans

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PubMed  57, 363–373 (1992).By suppressing a loss-of-function mutation that constitutively activates the vulval pathway, mutations in the activators of the pathway were obtained. Kemphues, K. J., Priess, J. R., Morton, D. G. & Cheng, N. Identification of genes required for cytoplasmic localization of early C. elegans embryos. Cold Spring Harb. Mango, S. E. Stop making nonsense: the C. elegans smg genes. Cell 99, 781–790 (1999). Trends Genet. Fay, D. S., Keenan, S. & Han, M. fzr-1 and lin-35/Rb function redundantly to control cell proliferation in C. elegans as revealed by a nonbiased synthetic screen. Two homologous regulatory genes, lin-12 and glp-1, have overlapping functions. 1 (eds Riddle, D. L., Blumenthal, T., Meyer, B. J. They are used as genetic tools because they allow lethal mutations to be propagated indefinitely. De Bono, M. & Bargmann, C. I. Genes Dev. Ambros, V. in C. elegans II (eds Riddle, D. L., Blumenthal, T., Meyer, B. J. Curr. Hodgkin, J. Crump, J. G., Zhen, M., Jin, Y. A motor neuron in the pharynx that is required for the peristaltic movements of the muscle that move food into the grinder. Dev. In addition, balancer chromosomes frequently contain rearrangements or translocations that disrupt recombination between the homologues. … Gen. Genet. Balancer chromosomes are used in trans to a chromosome that carries a lethal mutation. Nature Reviews Genetics Since 1974, when Sydney Brenner published his pioneering genetic screen, researchers have developed increasingly powerful methods for identifying genes and genetic pathways in C. elegans. The mec-8 gene of C. elegans encodes a protein with two RNA recognition motifs and regulates alternative splicing of unc-52 transcripts. Mol. ISSN 1471-0064 (online). USA 90, 7431–7435 (1993). Singh, N. & Han, M. sur-2, a novel gene, functions late in the let-60 ras-mediated signaling pathway during Caenorhabditis elegans vulval induction. 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